Cosmetic related Exosome Characterization Service

Creative Biolabs is a leading service provider that focuses on cosmetic products development. Now we are proud to provide the exosomes characterization service for our clients all over the world.

Introduction of Exosomes Characterization

In order to better determine their biological interactions, it is important to evaluate the physicochemical properties of exosomes, including size, shape, density, and porosity.

In recent years, there are a series of techniques have been developed to characterize exosomes, including biophysical, molecular, and microfluidic methods. Each of these techniques has its own unique advantages and limitations, so the choice needs to be made on a case-by-case basis.

Figure 1. Various techniques used for isolation, characterization, and analysis of functional properties of exosomes. (Gurunathan, 2021)

Nanoparticle Tracking Analysis (NTA)

Nanoparticle tracking analysis (NTA) is a biophysical approach to measure the size distribution and concentration of exosomes with the range of 10 nm to 2 µm. Each particle can be tracked through image analysis, and then the particle size, size distribution, concentration, and phenotype are finally output through the movement of exosomes. Sample preparation and measurements can be performed quickly and easily using this method. Furthermore, after performing the measurement, the sample can be recovered in its original form.

Dynamic Light Scattering

Dynamic light scattering (DLS), also known as photon correlation spectroscopy, is the technique to measure the size of exosomes. The technique measures particles from 1 nm to 6 µm in size by passing a monochromatic coherent laser beam through a suspension of particles to obtain time-varying fluctuations in scattering intensity. This technique is mainly used to measure a particle in suspension (monodisperse suspension).

Resistive Pulse Sensing

Resistive pulse sensing (RPS) is the novel technique to measure the size distribution and concentration of exosomes with approximately 50 nm to cell size. However, potential problems with this technique are susceptibility to system stability issues and insufficient sensitivity.

Atomic Force Microscopy

Atomic force microscopy (AFM) is the optical and electron diffraction technique to characterize the abundance, morphology, biomechanics, and biomolecular make-up of exosomes. This technique helps us understand exosomes at the single vesicle and subvesicle levels. However, it is important to note that the characterization of the samples by this method is performed from the external analysis.

Transmission Electron Microscopy

Transmission electron microscopy (TEM) is the technique widely used to characterize the structure, morphology, and size of various biological components. It has been specifically applied for the visualization of extracellular vesicles (EVs), and the obtained images can then be used for vesicle diameter measurements.

Flow Cytometry

Flow cytometry is a molecular method used to characterize exosome surface proteins. As a technique well suited for reproducible analysis of clinical samples, it can analyze different physical and chemical properties of cells and particles in suspension and can measure the size and structure of exosomes.

Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is confident in offering the most qualified services and products for our customers all over the world. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

Gurunathan, S.; et al. Review of the Isolation, Characterization, Biological Function, and Multifarious Therapeutic Approaches of Exosomes. Cells. 2021, 10(2): 462.