Cosmetic Exosomes Quantification Service

Creative Biolabs is a leading service provider that focuses on cosmetic products development. Now we are proud to provide the exosomes quantification service for our clients all over the world.

Introduction of Exosomes Quantification

Released by almost all cell types, exosomes are small vesicular structures with sizes ranging from 40-150 nm in diameter. In recent years, exosomes have been extensively studied as they can provide rich information in cellular physiology. Different from other classes of EVs, exosomes are formed as part of the endosomal pathway. In this case, exosomes quantification is essential to better understand the basic biological functions of exosomes. Multiple techniques have been developed to achieve exosomes quantification.

Figure 1. Schematic representation of the Nanosight NTA system. (Koritzinsky, 2017)

Nanosight Nanoparticle Tracking Analysis

Nanosight nanoparticle tracking analysis is the technology to count and size nanoparticles in a liquid suspension. While the detection of the specific marker is not required and exosomes can be suspended in various solutions, it has been served as the best method for exosomes quantification. However, it is important to note that samples must b diluted to a specific range of concentrations to obtain accurate and reproducible size distribution and data.

Vesicle Flow Cytometry

Flow cytometry (FC) allows the detection of particles suspended in a fluid via the interaction with a laser beam. Flow cytometers often need to be customized to meet the needs of exosome detection. However, flow cytometry is not sensitive in the smallest size range and is also challenging to determine particle size

Surface Plasmon Resonance

Surface plasmon resonance (SPR) can detect the binding of exosomes to the surface through changes in the optical properties of the surface. The advantage of this technique is the ability to quantify subpopulations of exosomes or individual proteins present in exosomes.

Electron Microscopy

Electron microscopy (EM) is a gold standard to verify the quality of exosome preparations. However, the labor-intensive, low-throughput nature of this technique can easily lead to exosome loss resulting in a significant underestimation of exosome numbers.

Advantages of our Exosomes Quantification Service

Higher operating efficiency
Suitable for a wide range of samples
High-quality with low cost
Fully customizable experimental design to expand beyond standard procedure.

Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is confident in offering the most qualified services and products for our customers all over the world. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

Koritzinsky, E.; et al. Quantification of exosomes. Journal of cellular physiology. 2017, 232(7): 1587-1590.