Cosmetic Exosomes Isolation Service

Creative Biolabs is a leading service provider that focuses on cosmetic products development. Now we are proud to provide the exosomes isolation service for our clients all over the world.

Introduction of Exosomes Isolation in Cosmetics

In order to facilitate the research and application of exosomes in cosmetic products, it is important to isolate exosomes specifically from cellular debris and interfering components.

Creative Biolabs has the ability to isolate exosomes from different sample matrices with high efficiency. The developed optical and non-optical techniques can be used to assess their size, morphology, quantity, and biochemical composition. Depending on the specific properties of exosomes, different isolation methods can be chosen.

Figure 1. Schematic representation of isolating exosomes by differential ultracentrifugation. (Li, 2017)

Ultracentrifugation-based Isolation

Centrifugation is the method to separate and purify particulate materials, and ultracentrifugation can generate ultra-high centrifugal forces of up to 1,000,000 × g making it the most commonly used standard method for exosome isolation. There are differential ultracentrifugation and density gradient ultracentrifugation. Differential ultracentrifugation adjusts centrifugal force and duration for exosome isolation based on the density and size differences between exosomes and other components in the sample. In isopycnic ultracentrifugation, exosomes are deposited along with the density gradient medium to a location with the same density as the medium, i.e., the isopycnic location.

Size-based Isolation

Similar to membrane filtration, ultrafiltration is size and molecular weight-based exosome isolation technique. Compared to ultracentrifugation, ultrafiltration is faster and requires no special equipment. What’s more, size exclusion chromatography (SEC) and flow field-flow fractionation are also size-based exosome isolation techniques.

Immunoaffinity capture-based Isolation

A large number of proteins and receptors present in exosomal membranes can serve as the basis for immunoaffinity capture. Typically, exosomal biomarkers for immunoisolation are membrane-bound, lack soluble counterparts, and are only expressed or highly concentrated on the surface of exosomes of specific biological origin.

Exosome Precipitation

Polymers that do not contain water, such as polyethylene glycol (PEG), bind water molecules and push less soluble components out of the solution. Therefore, by changing the solubility or dispersibility of exosomes, they can be precipitated from biological fluids. In addition, a variety of exosome precipitation kits have been developed on the market.

Microfluidics-based Isolation

Based on rapidly developing microfabrication techniques, fabricated microfluidic devices can utilize the physical and biochemical properties of microscale exosomes to rapidly and efficiently isolate exosomes. Significant reductions in sample volume, reagent consumption, and isolation time are expected through the use of such equipment.

Advantages of our Exosomes Isolation Service

Higher operating efficiency
Suitable for a wide range of samples
High-quality with low cost
Fully customizable experimental design to expand beyond standard procedure.

Equipped with world-leading technology platforms and professional scientific staff, Creative Biolabs is confident in offering the most qualified services and products for our customers all over the world. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

Li, P.; et al. Progress in exosome isolation techniques. Theranostics, 2017, 7(3): 789.