Introduction

Epidermal aging refers to the gradual decline in the structure and function of the epidermis, the outermost layer of the skin, because of intrinsic (chronological) and extrinsic (environmental) factors. Intrinsic aging is driven by genetic and metabolic processes, leading to reduced cell turnover, thinning of the epidermis, and impaired barrier function. Extrinsic aging, primarily caused by ultraviolet (UV) radiation, pollution, and lifestyle factors, accelerates these changes by inducing oxidative stress, DNA damage, and inflammation. There are two major types of epidermal aging: intrinsic aging, which is a natural biological process, and photoaging, which results from prolonged UV exposure. Hallmarks of epidermal aging include dryness, rough texture, uneven pigmentation, decreased regenerative capacity, and increased susceptibility to environmental insults. Creative Biolabs offers specialized services for studying the mechanism of epidermal aging, utilizing a wide array of in vitro and in vivo models that closely mimic human skin physiology. Our services include detailed assessments of cellular senescence, oxidative stress, gene and protein expression, and epidermal barrier function. These studies support the development and evaluation of anti-aging strategies targeting the epidermis.

Services

Creative Biolabs offers a comprehensive suite of well-established in vitro and in vivo models specifically designed for investigating the mechanisms of epidermal aging. Our models simulate both intrinsic and extrinsic aging processes, including reduced keratinocyte proliferation, impaired barrier function, oxidative stress, and UV-induced damage. These systems are carefully developed to replicate the structural and functional characteristics of human epidermis, enabling in-depth evaluations of how cosmetic ingredients impact skin aging at the epidermal level. Our expert research team provides end-to-end support throughout your project, from model selection and experimental design to data acquisition and interpretation. With advanced analytical techniques and a strong foundation in dermatological science, we ensure accurate, reproducible, and meaningful results. To learn more about our available models for studying the mechanisms of epidermal aging, please explore the links below.

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Measurements

We offer a diverse range of measurements for evaluating the mechanisms of epidermal aging, utilizing advanced in vitro and in vivo models that replicate the physiological and pathological changes of aged human skin. Our evaluation approach includes, but is not limited to:

• Morphological and Histological Analysis: Assessment of epidermal thickness, stratum corneum integrity, and cellular organization using H&E and immunohistochemical staining.
• Cellular Senescence Markers: Detection of aging-associated markers such as p16^INK4a, p21, and SA-β-Gal activity in keratinocytes.
• Barrier Function Evaluation: Measurement of transepidermal water loss (TEWL), hydration level, and lipid composition to assess skin barrier integrity.
• Oxidative Stress and DNA Damage: Quantification of reactive oxygen species (ROS), 8-OHdG, and antioxidant enzyme activity (e.g., SOD, catalase).
• Gene and Protein Expression Profiling: Analysis of aging-related gene and protein changes using RT-qPCR and Western blotting, including matrix metalloproteinases (e.g., MMP-1, MMP-9), filaggrin, involucrin, and collagen-related markers.
• Inflammatory Cytokine Profiling: ELISA or multiplex assays for pro-inflammatory mediators such as IL-6, IL-8, and TNF-α.

Beyond our established epidermal aging models, we provide customized model development based on the latest scientific literature and client-specific requirements. Our experienced scientific team supports every phase of your project, from experimental planning and method selection to comprehensive data analysis, ensuring a tailored and effective research strategy.

Advantages

1. Extensive Expertise: With years of experience in preclinical research and model development, we possess deep expertise in a wide range of therapeutic and cosmetic research areas, including skin aging, inflammation, oncology, and gastrointestinal diseases. Our scientific team stays at the forefront of emerging research trends to deliver cutting-edge solutions.

2. Comprehensive Model Systems: We offer a broad portfolio of well-established in vitro and in vivo models that closely mimic human physiology and pathology. Whether studying epidermal aging, wrinkle formation, or drug efficacy, our models enable precise, reproducible, and translatable results.

3. Customized Research Solutions: Understanding that every project is unique, we provide fully customized research services. From literature-based model development to tailored study design and endpoint analysis, we adapt to your specific scientific objectives and timelines.

4. Advanced Technology Platforms: Equipped with state-of-the-art technologies—including immunohistochemistry, gene/protein expression profiling, cytokine analysis, and high-throughput screening—we ensure comprehensive and high-quality data collection and interpretation.

5. Strict Quality Control & Regulatory Compliance: Our rigorous quality assurance protocols and adherence to international standards guarantee reliable and reproducible outcomes, making us a trusted partner for both academic and industry clients.

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Published Data

Reference

1. Nedachi, Taku et al. "Chronological aging impacts abundance, function and microRNA content of extracellular vesicles produced by human epidermal keratinocytes." Aging vol. 15,22 (2023): 12702-12722. DOI:10.18632/aging.205245. Distributed under Open Access license CC BY 4.0, without modification.

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