Creative Biolabs is an internationally operating testing laboratory that provides a series of toxicology test services for cosmetic products. Our comprehensive safety testing capabilities meet the international regulatory requirements for cosmetic products. Now we provide multiple methods for carcinogenicity testing.

The Introduction of Carcinogenicity

Carcinogenesis is a complex multistage process to transform normal cells into cancer cells. The changes always occur in different stages and can be divided into four different steps, which include preventing cell differentiation, obtaining immortality, tumorigenicity, as well as complete malignancy, and metastasis. Certain chemicals can induce carcinogenesis by initiating and promoting. If a chemical substance can be shown to have an effect on any of these stages, it is considered a potential carcinogen. Although in vitro models are unlikely to fully simulate the real environment because of the complexity of carcinogenicity, information about hazards and risks can be obtained to a certain extent.

Figure 1. Examples of normal and morphologically transformed SHE colonies after 7 days growth and stained in Giemsa. (Pickles, 2016)

Methods for Carcinogenicity Test

• Bhas 42 Cell Transformation Assay (CTA)

The in vitro Bhas 42 Cell Transformation Assay (CTA) is the novel method to assess the carcinogenic potential of cosmetic products. This method uses a cell line with the ability to reproduce the multi-stage cell transformation process that occurs when normal cells transform into cancer cells. The detection system is composed of two parts, which respectively evaluate whether the chemical substance will have an initiating effect or a promoting effect. We can use both the standard 6-well method and the 96-well method to perform this assay.

• SyrianHamster Embryo (SHE) Assay

The SyrianHamster Embryo (SHE) Assay is short-term in vitro assay to predict carcinogenicity of cosmetic products with a high degree of concordance. This method aims to evaluate the effects of chemicals on the growth of specific cell types and the transformation process from normal cells to fully malignant cells. This assay involves exposing cells to a test chemical for a specific length of time and then using an optical microscope to visually score them based on specific parameters related to phenotype and growth patterns. Compared with other short-term in vitro assays, both genotoxic and epigenetic carcinogens can be detected.

Advantages of our Carcinogenicity Test

• No uses animals to avoid ethical and legal issues.
• Able to meet regulatory requirements.
• Customized services to meet various needs.
• Low price with good service.

Equipped with world-leading technology platforms and professional scientific staff, we are pleased to offer the most qualified services and products for our customers all over the world. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

Reference

1. Pickles, J.; et al. A mechanistic evaluation of the Syrian hamster embryo cell transformation assay (pH 6.7) and molecular events leading to senescence bypass in SHE cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2016, 802: 50-58.

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