Creative Biolabs is an internationally operating testing laboratory that provides a series of toxicology test services for cosmetic products. Our comprehensive safety testing capabilities meet the international regulatory requirements for cosmetic products. Now we provide the novel method for phototoxicity testing.

The Introduction of Phototoxicity Test

Phototoxicity, also known as photoirradiation, is defined as the toxic response induced upon initial exposure of skin to a test sample, and subsequent exposure to light. It is a type of photosensitivity requiring light but does not involve the immune system. In addition, it also involves toxic reactions induced by skin irradiation after systemic administration (oral, intravenous) of a chemical substance. If the chemical absorbs ultraviolet or visible light, the absorbed energy may cause molecular changes, causing the chemical to become toxic. For example, various synthetic compounds, including tetracyclines or fluoroquinolones, are known to cause these effects.

In Vitro 3T3 BRU Phototoxicity Test (OECD 432)

If a cosmetic product absorbs UV or visible light, it would be necessary to determine if it is likely to cause adverse phototoxic effects when intended for human use.

The in vitro 3T3 BRU Phototoxicity test is a sensitive approach to evaluate the potential of a chemical agent to induce phototoxicity. This test system contains the immortalized mouse fibroblast cell line, Balb/c 3T3. Using 3T3 cells, this method evaluates cytotoxicity by the uptake of neutral red after exposure to the test substance. This difference in absorption can be measured with a spectrophotometer, which also allows the distinction and quantification between viable, damaged, and dead cells. What’s more, this method has been adopted as OECD test guideline No 432 to predict acute phototoxicity effects in animals and humans in vivo.

Advantages of our Phototoxicity Testing

• No uses animals to avoid ethical and legal issues.
• Extensive experience and advanced biotechnologies.
• Competitive price with the best quality.
• Fully customizable experimental design to expand beyond standard procedure.

Equipped with world-leading technology platforms and professional scientific staff, we are pleased to offer the most qualified services and products for our customers all over the world. If you are interested in our products or services, please do not hesitate to contact us for more detailed information.

• Cytotoxicity Test
• Skin Irritation/Corrosion Test
• Eye Irritation/Corrosion Test
• Skin Sensitization Test
• Genetic Toxicity Test
• Skin Penetration Test
• Endocrine Disruptor Activity Test
• Reproductive Toxicity Test
• Carcinogenicity Test